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nppc commercial elisa novus bio  (Novus Biologicals)


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    Novus Biologicals nppc commercial elisa novus bio
    Figure 6. Identification and validation of new secreted estrous staging markers. (A) Expression of granulosa cell transcripts varying by estrous cycle stage. (B) Validation of significantly up- and downregulated transcripts of secreted estrous staging markers by qPCR (n=5 per group, mean ± SEM, *p<0.05, **p<0.01, and ***p<0.005). (C) Localization of estrous staging markers by in situ hybridization (RNAscope) in ovarian sections. (D) Quantification of circulating estrous staging markers proteins in the blood by enzyme-Linked Immunosorbent assay <t>(ELISA)</t> (n=5 per group, mean ± SEM, *p<0.05, and ***p<0.005). (E) Summary of the timing of expression of estrous staging markers in the blood.
    Nppc Commercial Elisa Novus Bio, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nppc+commercial+elisa+novus+bio/Mouse+CNP%2FNPPC+ELISA+Kit+(Colorimetric)/10__7554_slash_elife__77239-213-50-53
    Average 91 stars, based on 2 article reviews
    nppc commercial elisa novus bio - by Bioz Stars, 2026-09
    91/100 stars

    Images

    1) Product Images from "A single-cell atlas of the cycling murine ovary"

    Article Title: A single-cell atlas of the cycling murine ovary

    Journal: eLife

    doi: 10.7554/elife.77239

    Figure 6. Identification and validation of new secreted estrous staging markers. (A) Expression of granulosa cell transcripts varying by estrous cycle stage. (B) Validation of significantly up- and downregulated transcripts of secreted estrous staging markers by qPCR (n=5 per group, mean ± SEM, *p<0.05, **p<0.01, and ***p<0.005). (C) Localization of estrous staging markers by in situ hybridization (RNAscope) in ovarian sections. (D) Quantification of circulating estrous staging markers proteins in the blood by enzyme-Linked Immunosorbent assay (ELISA) (n=5 per group, mean ± SEM, *p<0.05, and ***p<0.005). (E) Summary of the timing of expression of estrous staging markers in the blood.
    Figure Legend Snippet: Figure 6. Identification and validation of new secreted estrous staging markers. (A) Expression of granulosa cell transcripts varying by estrous cycle stage. (B) Validation of significantly up- and downregulated transcripts of secreted estrous staging markers by qPCR (n=5 per group, mean ± SEM, *p<0.05, **p<0.01, and ***p<0.005). (C) Localization of estrous staging markers by in situ hybridization (RNAscope) in ovarian sections. (D) Quantification of circulating estrous staging markers proteins in the blood by enzyme-Linked Immunosorbent assay (ELISA) (n=5 per group, mean ± SEM, *p<0.05, and ***p<0.005). (E) Summary of the timing of expression of estrous staging markers in the blood.

    Techniques Used: Biomarker Discovery, Expressing, In Situ Hybridization, RNAscope, Enzyme-linked Immunosorbent Assay

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: A single-cell atlas of the cycling murine ovary
    Article Snippet: .. Reagent type (species) or resource Designation Source or reference Identifiers Additional information Genetic reagent (Mus musculus) C57BL/6- Tg(UBC- GFP)30Scha/J Jackson Laboratory stock #004353 Antibody Smooth muscle alpha action (SMA) (Rabbit polyclonal) Abcam #5694 Dilution: 1:300 Commercial assay or kit ACTIVIN A commercial ELISA RnD systems #DAC00B Commercial assay or kit NPPC commercial ELISA Novus Bio #NBP2- 75790 Commercial assay or kit Tinagl1 commercial ELISA LS- Bio #LS- F49684 Commercial assay or kit PRSS35 commercial ELISA Mybiosource #MBS9717242 Commercial assay or kit RNA scope 2.5 HD Duplex detection kit ACD bio #322500 Commercial assay or kit RNA scope 2.5 HD red detection kit ACD bio #322360 Commercial assay or kit The target retrieval and protease plus reagents ACD bio #322330 Other Cdkn1a (M. musculus) NM_007669.4 ACD bio # 408551 RNAscope probe Other Cxcl14 (M. musculus) NM_019568.2 ACD bio #459741 RNAscope probe Morris, Meinsohn et al. eLife 2022;11:e77239. .. DOI: https://doi.org/10.7554/eLife.77239 15 of 24 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Other Cyp11a1 (M. musculus) NM_019779.4 ACD bio #809181 RNAscope probe Other Cyp17a1 (M. musculus) NM_007809.3 ACD bio #522611 RNAscope probe Other Ghr (M. musculus) NM_010284.3 ACD bio #464951 RNAscope probe Other Hhip (M. musculus) NM_020259.4 ACD bio #448441 RNAscope probe Other Inhba (M. musculus) NM_008380.1 ACD bio # 455871 RNAscope probe Other Kcnk2 (M. musculus) NM_001159850.1 ACD bio #440421 RNAscope probe Other Kctd14 (M. musculus) NM_001136235.1 ACD bio #517811 RNAscope probe Other Mfap5 (M. musculus) NM_015776.2 ACD bio #490211 RNAscope probe Other Mro (M. musculus) NM_001305882.1 ACD bio #491181 RNAscope probe Other Neat1 (M. musculus) NR_003513.2 ACD bio #440351 RNAscope probe Other Nppc (M. musculus) NM_010933.5 ACD bio # 493291 RNAscope probe Other Onecut2 (M. musculus) NM_194268.2 ACD bio #520541 RNAscope probe Other Oxtr (M. musculus) NM_001081147.1 ACD bio #412171 RNAscope probe Other Prss35 (M. musculus) NM_178738.3 ACD bio #492611 RNAscope probe Other Runx1 (M. musculus) NM_001111021.1 ACD bio #406671 RNAscope probe Other Tinagl1 (M. musculus) NM_001168333 ACD bio #312621 RNAscope probe Other Top2a (M. musculus) NM_011623.2 ACD bio # 491221 RNAscope probe Other Wt1 (M. musculus) NM_144783.2 ACD bio #432711 RNAscope probe Software and algorithm R version 4.1.3 R Project for Statistical Computing https://scicrunch.org/resolver/SCR_ 001905 Software and algorithm Seurat package 4.1.0 R toolkit for single- cell genomics https://satijalab.org/seurat/articles/ install.html Software and algorithm BZ- X800 analysis software Keyence https://www.keyence.com/landing/ microscope/lp_fluorescence.jsp Software and algorithm GraphPad Prism, version 9.2.0 Graphpad Continued

    RNAscope:

    Article Title: A single-cell atlas of the cycling murine ovary
    Article Snippet: .. Reagent type (species) or resource Designation Source or reference Identifiers Additional information Genetic reagent (Mus musculus) C57BL/6- Tg(UBC- GFP)30Scha/J Jackson Laboratory stock #004353 Antibody Smooth muscle alpha action (SMA) (Rabbit polyclonal) Abcam #5694 Dilution: 1:300 Commercial assay or kit ACTIVIN A commercial ELISA RnD systems #DAC00B Commercial assay or kit NPPC commercial ELISA Novus Bio #NBP2- 75790 Commercial assay or kit Tinagl1 commercial ELISA LS- Bio #LS- F49684 Commercial assay or kit PRSS35 commercial ELISA Mybiosource #MBS9717242 Commercial assay or kit RNA scope 2.5 HD Duplex detection kit ACD bio #322500 Commercial assay or kit RNA scope 2.5 HD red detection kit ACD bio #322360 Commercial assay or kit The target retrieval and protease plus reagents ACD bio #322330 Other Cdkn1a (M. musculus) NM_007669.4 ACD bio # 408551 RNAscope probe Other Cxcl14 (M. musculus) NM_019568.2 ACD bio #459741 RNAscope probe Morris, Meinsohn et al. eLife 2022;11:e77239. .. DOI: https://doi.org/10.7554/eLife.77239 15 of 24 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Other Cyp11a1 (M. musculus) NM_019779.4 ACD bio #809181 RNAscope probe Other Cyp17a1 (M. musculus) NM_007809.3 ACD bio #522611 RNAscope probe Other Ghr (M. musculus) NM_010284.3 ACD bio #464951 RNAscope probe Other Hhip (M. musculus) NM_020259.4 ACD bio #448441 RNAscope probe Other Inhba (M. musculus) NM_008380.1 ACD bio # 455871 RNAscope probe Other Kcnk2 (M. musculus) NM_001159850.1 ACD bio #440421 RNAscope probe Other Kctd14 (M. musculus) NM_001136235.1 ACD bio #517811 RNAscope probe Other Mfap5 (M. musculus) NM_015776.2 ACD bio #490211 RNAscope probe Other Mro (M. musculus) NM_001305882.1 ACD bio #491181 RNAscope probe Other Neat1 (M. musculus) NR_003513.2 ACD bio #440351 RNAscope probe Other Nppc (M. musculus) NM_010933.5 ACD bio # 493291 RNAscope probe Other Onecut2 (M. musculus) NM_194268.2 ACD bio #520541 RNAscope probe Other Oxtr (M. musculus) NM_001081147.1 ACD bio #412171 RNAscope probe Other Prss35 (M. musculus) NM_178738.3 ACD bio #492611 RNAscope probe Other Runx1 (M. musculus) NM_001111021.1 ACD bio #406671 RNAscope probe Other Tinagl1 (M. musculus) NM_001168333 ACD bio #312621 RNAscope probe Other Top2a (M. musculus) NM_011623.2 ACD bio # 491221 RNAscope probe Other Wt1 (M. musculus) NM_144783.2 ACD bio #432711 RNAscope probe Software and algorithm R version 4.1.3 R Project for Statistical Computing https://scicrunch.org/resolver/SCR_ 001905 Software and algorithm Seurat package 4.1.0 R toolkit for single- cell genomics https://satijalab.org/seurat/articles/ install.html Software and algorithm BZ- X800 analysis software Keyence https://www.keyence.com/landing/ microscope/lp_fluorescence.jsp Software and algorithm GraphPad Prism, version 9.2.0 Graphpad Continued



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    Figure 6. Identification and validation of new secreted estrous staging markers. (A) Expression of granulosa cell transcripts varying by estrous cycle stage. (B) Validation of significantly up- and downregulated transcripts of secreted estrous staging markers by qPCR (n=5 per group, mean ± SEM, *p<0.05, **p<0.01, and ***p<0.005). (C) Localization of estrous staging markers by in situ hybridization (RNAscope) in ovarian sections. (D) Quantification of circulating estrous staging markers proteins in the blood by enzyme-Linked Immunosorbent assay <t>(ELISA)</t> (n=5 per group, mean ± SEM, *p<0.05, and ***p<0.005). (E) Summary of the timing of expression of estrous staging markers in the blood.
    Nppc Commercial Elisa Novus Bio, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nppc+commercial+elisa+novus+bio/Mouse+CNP%2FNPPC+ELISA+Kit+(Colorimetric)/10__7554_slash_elife__77239-213-50-53
    Average 91 stars, based on 1 article reviews
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    nppc commercial elisa novus bio  (R&D Systems)
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    Figure 6. Identification and validation of new secreted estrous staging markers. (A) Expression of granulosa cell transcripts varying by estrous cycle stage. (B) Validation of significantly up- and downregulated transcripts of secreted estrous staging markers by qPCR (n=5 per group, mean ± SEM, *p<0.05, **p<0.01, and ***p<0.005). (C) Localization of estrous staging markers by in situ hybridization (RNAscope) in ovarian sections. (D) Quantification of circulating estrous staging markers proteins in the blood by enzyme-Linked Immunosorbent assay <t>(ELISA)</t> (n=5 per group, mean ± SEM, *p<0.05, and ***p<0.005). (E) Summary of the timing of expression of estrous staging markers in the blood.
    Nppc Commercial Elisa Novus Bio, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nppc+commercial+elisa+novus+bio/Mouse+CNP%2FNPPC+ELISA+Kit+(Colorimetric)/10__7554_slash_elife__77239-213-50-43
    Average 91 stars, based on 1 article reviews
    nppc commercial elisa novus bio - by Bioz Stars, 2026-09
    91/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 6. Identification and validation of new secreted estrous staging markers. (A) Expression of granulosa cell transcripts varying by estrous cycle stage. (B) Validation of significantly up- and downregulated transcripts of secreted estrous staging markers by qPCR (n=5 per group, mean ± SEM, *p<0.05, **p<0.01, and ***p<0.005). (C) Localization of estrous staging markers by in situ hybridization (RNAscope) in ovarian sections. (D) Quantification of circulating estrous staging markers proteins in the blood by enzyme-Linked Immunosorbent assay (ELISA) (n=5 per group, mean ± SEM, *p<0.05, and ***p<0.005). (E) Summary of the timing of expression of estrous staging markers in the blood.

    Journal: eLife

    Article Title: A single-cell atlas of the cycling murine ovary

    doi: 10.7554/elife.77239

    Figure Lengend Snippet: Figure 6. Identification and validation of new secreted estrous staging markers. (A) Expression of granulosa cell transcripts varying by estrous cycle stage. (B) Validation of significantly up- and downregulated transcripts of secreted estrous staging markers by qPCR (n=5 per group, mean ± SEM, *p<0.05, **p<0.01, and ***p<0.005). (C) Localization of estrous staging markers by in situ hybridization (RNAscope) in ovarian sections. (D) Quantification of circulating estrous staging markers proteins in the blood by enzyme-Linked Immunosorbent assay (ELISA) (n=5 per group, mean ± SEM, *p<0.05, and ***p<0.005). (E) Summary of the timing of expression of estrous staging markers in the blood.

    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Genetic reagent (Mus musculus) C57BL/6- Tg(UBC- GFP)30Scha/J Jackson Laboratory stock #004353 Antibody Smooth muscle alpha action (SMA) (Rabbit polyclonal) Abcam #5694 Dilution: 1:300 Commercial assay or kit ACTIVIN A commercial ELISA RnD systems #DAC00B Commercial assay or kit NPPC commercial ELISA Novus Bio #NBP2- 75790 Commercial assay or kit Tinagl1 commercial ELISA LS- Bio #LS- F49684 Commercial assay or kit PRSS35 commercial ELISA Mybiosource #MBS9717242 Commercial assay or kit RNA scope 2.5 HD Duplex detection kit ACD bio #322500 Commercial assay or kit RNA scope 2.5 HD red detection kit ACD bio #322360 Commercial assay or kit The target retrieval and protease plus reagents ACD bio #322330 Other Cdkn1a (M. musculus) NM_007669.4 ACD bio # 408551 RNAscope probe Other Cxcl14 (M. musculus) NM_019568.2 ACD bio #459741 RNAscope probe Morris, Meinsohn et al. eLife 2022;11:e77239.

    Techniques: Biomarker Discovery, Expressing, In Situ Hybridization, RNAscope, Enzyme-linked Immunosorbent Assay